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46
| 1 | 2008
Klinisk Biokemi i Norden
Abstract
The new easy particle-enhanced immunoturbidi-
metric method from Thermo Fisher Scientific for
measuring myoglobin seems to be well suited to
daily clinical use with Konelab-family automated
chemistry analyzers, or with other analyzers allowing
the use of user-defined applications and non-system
reagents.
Introduction
Myoglobin, with a molecular weight of 17.8 kD, is
an oxygen-binding protein located in skeletal and
cardiac muscle tissues. Myoglobin is rapidly released
into the bloodstream in various types of tissue inju-
ry, including myocardial infarction. The change in
myoglobin concentration in the blood can be used
as a marker for various tissue and organ injuries, and
also as a rapid, non-specific cardiac marker to exclude
myocardial damage, together with more specific car-
diac proteins with elevated concentrations later on
after the injury [1].
The purpose of this study was to evaluate the new
particle-enhanced immunoturbidimetric method of
myoglobin in Konelab™ analysers, and the evaluation
was conducted on a Konelab™ 20XTi analyzer pro-
duced by Thermo Fisher Scientific. This instrument
is widely used in small clinical laboratories. Results
obtained from surplus human plasma samples were
compared to another commercial electrochemilumi-
nescence assay of myoglobin at normal and elevated
concentrations of myoglobin.
The preliminary results were presented at the
XXX Nordic Congress of Clinical Chemistry in
Copenhagen, Denmark 14-17 June 2006.
Easily Applicable Myoglobin Method on the
Konelab™ Analyzer
Ilkka M. Penttilä
1
, Kari Savolainen
2
, Hannu Lampinen
3
, Jouko Lukkarinen
3
1
Kuopio Research Institute of Exercise Medicine, Kuopio, Finland
2
Laboratory Centre, Kuopio University Hospital, Kuopio, Finland
3
Thermo Fisher Scientific Co., Vantaa, Finland
E-post: ilkka.penttila@pp.inet.fi
Materials and Methods
Instruments
Konelab™ 20XTi Clinical chemistry analyzer (Thermo
Fisher Scientific Oy, Vantaa, Finland [2]) and
Roche Elecsys® 2010 Immunoassay analyzer (Roche
Diagnostics GmbH, Mannheim, Germany) [3].
Reagents
Konelab™ Myoglobin reagent (cat # 981 847)
Konelab™ Myoglobin Calibration Set
(cat # 981 849).
Konelab™ Myoglobin Control Low
(cat # 981 847) and High (cat # 981 847).
Roche Myoglobin STAT reagent
(cat # 11820788122)
Roche Myoglobin STAT Cal Set (cat # 11820893122)
Roche PreciControl Cardiac (cat # 03530477190)
Application
The application is based on a latex particle-enhanced
immunoturbidimetric measurement using polyclonal
anti-myoglobin antibody. The application starts with
the incubation of the reagent buffer and sample (10
µl) for 300 s, followed by a blank measurement and
addition of the antiserum. The antigen-antibody
reaction takes place during an incubation of 600 s,
after which an end-point measurement is done at
700 nm.
Results are calculated using a calibration curve
based on 0.9% NaCl and four separate product-
specific calibrators between 65 and 520 µg/l with
the measuring range of 40 - 520 µg/l [2]. No inter-
ference to myoglobin values with bilirubin is found
