4.2 Calculation at eonstant Pathlength

Symbol Type of Quantity

b

Catalytic activity concentration

i

Increase of 410nm absorbance per

seeond

~

Molar 410 rum absorption of

5-amino-2-nitrobenzoate

=

790.8 (example)

l

Light pathlength (0.01)

v

Sample volume fraction: 0.0909

vol (L) /vol (L)

4.2.1 Calculation for a Pathlength of 0.01 m

i

i

b

=

=

Ex

l

x

v

790.8

x

0.01

x

0.091

i

b

=

=

l.

391

0.7189

k at/m

3

b

=

i

x l.

391

x

b

=

i x 1391 x pkat/L

Calculated for a measuring time of 60s:

b

=

i x 1391 x pmol x (60s)-l x L-l

b

=

i x 1391 U/L

b

=

i x 23.18 pkat/L

x

x

Units

ykat/L

-l

s

m2 x mol-l

m

l

-l

s

2

-l

m

x

mol

x

-l

mol

x

s

3

m

To convert from y.kat / L to U/ L multiply by 60.

To convert from U/L to ykat / L divide by 60.

m

4.2.2 Calculation for Other Pathle9gths and for Other Volume

Fractions

Insert the pathlength (unit: meter) and the volume fraction

in the equation instead of the figures given above.

i

85

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