l. REACTION PRINCIPLEs
L-garnma-glutamyl-3-carboxy-4-nitroanilide
(L-Glucana)
and
glycylglycine (Glygly) are used as the donor and accepter
substrates, respectively.
The following reaction is catalyzed by human gamma-glutamyl–
transferase (GT) in serum:
L- - glutamyl–
3-carboxy-
f\
4-nitroanilide
5-Amino-
l
2-nitrobenzoate
l
/ 1
Glycylglycine
l
' L- -glutamyl-
~'
glycylglycine
For details of the reaction kinetics refer to the IFCC docu–
ment, ref.l.
Two hydrolysis and auto-transfer side reactions contribute
lessthan l% of the products (l).
Klinisk kemi
i
Norden 2: suppl. 1990
77
