Table 2
Concentrations are specified in the final reaction mixture at
o
37 C, to ensure that maximal GT catalytic activity will be
o
o
supported at both 37 C and 30
c.
Stage A. Incubation with reagent A
Volume fraction:
in incubation stage A
0.889
in complete reaction mixture
0.727
Example of
volume
(uL)
Glycylglycine-NaOH
buffer, pH 7.7
150 mmol/L
400
pL
Add sample. (Final) Volume fraction
0.091
50
pL
Mix well. Incubate until the reaction tempera-
ture has been reached.
Stage B.
Substrate Start with Reagent B:
Volume fraction
0.182
L-gamma-glutamyl-3-carboxy-4-nitroanilide
L-Glucana
6
mmol/L
100
pL
Mix well. Monitor for the minimum time and
number of data points to obtain acceptable
precision around the upper reference limit.
Klinisk kemi
i
Norden 2: suppl, 1990
81
l
