NCCLS and equivalent guideli nes
o~
Method Comparison should
be followed. (Cf refs 23- 24a, CK).
o
o
2.2 Measurements at 37 C and 30 C
The ECCLS standard Method performs equally well at either
reaction temperature. In routine use and quality assessments
results should be reported at the temperature at which the
measurements were made.
2.3 Temperature Conversion Factors
However, as discussed in the General Introduction, conversion
factors may be of use for cornparison of literature data.
In evaluating such data the choice of the calibration rnateri–
al and isoenzyrne composition must be considered. As an examp–
le, in Switzerland and in The Netherlands the average tempe-
o
o
rature conversion factors 37 C/ 37 C are 1.50 and 1.42, re-
spectively. (13,
s;.
2.4 Concentrations of Components in the Reaction Mixture
The concentrations in the complete reaction mixture depend
not only on the amounts in the original solution(s) but also
on the relative volumes of different solutions used to achie–
ve the final reaction mixture, as well as on the relative
volume of sample. Cf the discussion
2.4
in the CK Method.
2.5 Minimum Time of Saturation with Pyridoxal-5'-Phosphate
For the great majority of samples complete
activatio~
is
reached through incubation of sarnples with P-5'-p for 5 minu–
tes during the heating to the reaction temperature (7
13,
17 -
20).
62
Klinisk kemi
i
Norden 2: supp/, 1990
